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Pathological Changes and Molecular Detection of Avian Nephritis Virus from Commercial Broiler Chicken

Sathiyaseelan Mani; Kumar Raja; Avinash Warundeo Lakkawar; Madhavan Gopalakrishnan Nair; Thanislass Jacob; Khub Chandra Varshney.

Abstract
Avian nephritis virus (ANV) has been known to infect chickens causing a disease condition characterized by diarrhoea, retarded growth, tubulonephrosis, interstitial nephritis, uricosis (gout) and even death. The present study reports pathomorphology and detection of ANV by reverse transcriptase polymerase chain reaction (RT-PCR) in commercial broiler from Puducherry and Tamilnadu, India during March 2015 and October 2015. Gout was recorded in 28/120 (23.33%) cases. The total number of 18 samples screened for ANV, 2 (11.11%) samples was found to be positive for RT-PCR. On microscopical examination of kidney large to moderate, focal to multifocal interstitial mononuclear cells infiltration were observed. Since the isolation and identification of ANV is complicated and time consuming the RT-PCR used in the present study can be a potential diagnostic tool for the cases of gout and nephropathy in chickens.

Key words: Gout;Pathology;Avian Nephritis Virus;RT-PCR;Broiler Chicken



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