The measurement of plasma melatonin levels by high performance liquid chromatographyEsin Ozkan, Halil Yaman, Erdinc Cakir, Ibrahim Aydin, Muzaffer Oztosun, Mehmet Agilli, Yasemin Gulcan Kurt, Emin Ozgur Akgul, Tuncer Cayci, Nevin Ilhan, Necip Ilhan.
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Objective: We aimed to establish high performance liquid chromatography (HPLC) method to measure plasma melatonin levels easily, sensitively and reliably.
Methods: We used fluorescence detection, 75 mM sodium acetate/acetonitrile (72/28, v/v)(pH 5) as a mobile phase, isocratic method, 275 and 345 nm excitation and emission wavelengths, respectively. Samples were extracted using solid phase extraction column (Waters Oasis HLB 1 cc cartridge).
Results: The recovery was found as 97.2%. Inter-assay and intra-assay coefficients of variation (CV) were 4.02% and 5.41%, respectively. Detection limit was 0.25 pg/mL and linear range was up to 1000 pg/mL.
Conclusion: These findings suggest that our method is suitable to measure plasma melatonin levels.
HPLC; Melatonin; SPE
American Journal of Physiology, Biochemistry and Pharmacology
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