Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Interleukin-6 (-174G>C) promoter polymorphism in patients infected by hepatitis B virus

Serap Yalcin, Seref Demirbas, Onder Onguru.

Abstract
Objective: Hepatitis B virus (HBV) infection is one of the most important health problems in the world. Interleukin-6 (IL-6) has been shown to be a major inflammatory cytokine, inducing cell proliferation and expression of acute response genes, such as fibrinogen and C-reactive protein in hepatocytes. IL-6 levels are elevated in patients acutely infected with HBV and have been associated with progression of infection to chronic hepatitis.
Methods: In the present study, the role of IL-6 (-174G/C) polymorphism was investigated on individuals which are diagnosed as hepatitis B virus carrier (n=19), chronic hepatitis (n=10) and cirrhosis (n=13), and non-infected individuals with HBV (n=8). In order to determine IL-6 polymorphism, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied using genomic DNA extracted from paraffin embedded liver needle biopsy specimens.
Results: The genotype frequencies in 50 samples were observed as follows: 76% homozygote typical (GG), 22% heterozygote (GC) and 2% homozygote atypical (CC). In this study, limited statistical association was observed between IL-6 polymorphism (GG, GC and CC genotype) and chronic hepatitis/cirrhosis compared to hepatitis B virus carriers and non-infected individuals with HBV (p=0.045).
Conclusion: As observed in only one patient with chronic hepatitis, IL-6 (-174C/C) genotype is very rare in this study group. Because of lower frequency of -174C/C phenotype, studies in larger series can give statistically more precise conclusions.

Key words: Cytokine; Hepatitis B; Interleukin-6; Liver; Polymorphism



Share this Article


Advertisement
Journal of Complementary Medicine Research

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons