To evaluate collection Efficacy of cell separator machines and post transplant outcome of stem cell therapy collected from peripheral blood in various haematological conditions. Present study of 170 patients who underwent autologus and allogenic stem cell transplant collected from peripheral blood of both adult and paediatric age groups from year 1999 to 2012. Total 308 procedures were done among which 72(23%) were on Cs 3000 plus and rest on Amicus cell separator. 218 procedures were autologus and 90 were allogenic procedures. Mononuclear cell collection protocols on cell separators were adopted for harvesting PBSCs with the help of aphaeresis catheter. Mean collection efficacy of the Amicus aphaeresis machine is 75% and median yield is 6.6x108 MNC/Kg body weight. A median of 5.6x106 CD 34+ cells per kg recipient body weight were collected. On CS 3000 plus average collection efficacy was 50 % and yield 5.54x108 MNC/Kg body weight. 2.1x106 CD 34+ cells per kg body weight were harvested in a median product volume 50 ml. Post transplant median time for neutrophilic and platelet recovery is 12 days and 16 days respectively. Only 2 patients of Aplastic anemia and 2 patients of thalassemia had primary graft rejection. 6 patients died due to GVHD grade IV. Other patients had low grade reversible GVHD. Collection of PBSCs using the Amicus cell separator allows adequate yields of MNCs and CD34+ cells. Though the values of MNC count and CD 34+ cells are lower in CS3000 plus, outcome of engraftment was not affected. Engraftment takes place in more than 95% of the patients. Stem cell transplant is boon to the patients where other treatment is not responding or available.
Engraftment, graft versus host disease, mononuclear cell (MNC), Peripheral blood stem cell