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Investigation of in vitro Mineral Forming Bacterial Isolates from Subgingival Calculus

Turgut Demir, Ozlem Baris, Esra Zor.

Abstract
The mechanism that dictates the formation of dental calculus, an important component of periodontal health, has yet to be sufficiently explained. The effect of bacterial flora on dental calculus formation is important for periodontal status. Therefore, this study aims to investigate the mineral forming bacteria from subgingival calculus under anaerobic conditions.
Bacteria have been isolated from subgingival calculus, and isolates were examined for mineralization under anaerobic conditions. Bacterial isolates capable of crystal formation on plates were identified by their 16S rDNA sequences.
Bacterial isolates from subgingival dental calculus that form minerals in vitro have been identified as Streptococcus spp.
This is the first report to identify and show that bacteria from subgingival calculus under anaerobic conditions are involved in the formation of dental calculus.

Key words: Subgingival dental calculus, streptococcus, biomineralization, 16S rDNA sequence.



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