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Comparative study of assay of free radical damage in in vivo, in vitro and ex vivo conditions

Amit Singh, Surendra Pratap Mishra, Abha Mishra.

Abstract
Background: Interception of free radical events and damage in clinical situation remain arbitrary with poor understanding on antioxidant pharmacology.
Methods: Experiments on in vitro per-oxidation insult as well as assumed free radical damage situation in ischemia reperfusion are examined for profile of malondialdehyde rise and effect of Ginkgobiloba treatment by different schedule.
Results: Effect of Ginkgobiloba was seen on ischemia reperfusion induced, hydrogen peroxide induced and ferric chloride induced methods. Antioxidant effect of Ginkgobiloba was consistent in all techniques.
Conclusions: Results reveal the significance of complementary experiment to elaborate issue of antioxidants.

Key words: Antioxidant, Ginkgobiloba, Malondialdehyde



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