Plant tissue culture techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant. Single cells, plant cells without cell walls (protoplasts), pieces of leaves, stems or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Cultured plant cells can be offered alternative sources over whole plant cultivation for the production of secondary metabolites. The explants, such as plant leaves, stems, roots, meristems etc. were used for cultivation and establishment of plant cell and tissue cultures. Unfortunately, most often plant cell cultures technologies not lead to an enhancement in secondary metabolites production. Therefore, Strain improvement, methods for the selection of high-producing cell lines, and medium optimizations must be considered to improve the production of secondary metabolites. Several types of cell culture methods are being used to produce bioactive compounds. Cell suspension cultures are preferred for production large amounts of cells for quantitative or qualitative analytical studies. This review refers to certain bioactive secondary metabolites produced in the authorís laboratory by different tissue cultures techniques.
Key words: Cell suspension cultures, shoot cultures, hairy root cultures, bioactive compounds.