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J Pak Med Assoc. 2016; 66(4): 368-372


In vitro effects of nicotine on the non-small-cell lung cancer line A549

Tao Gao, Xue-Liang Zhou, Sheng Liu, Chang-Xiu Rao, Wen Shi, Ji-Chun Liu.

Abstract
Objective: To investigate in vitro effects of nicotine on the non-small-cell lung cancer line A549.
Methods: The case-control study was conducted at the First Affiliated Hospital of Nanchang University from 1st January to 30th June, 2014 and comprised A549 cells which were treated with a series of concentrations of nicotine (0.01 然, 0.1 然, 1 然 and 10 然) for 24 hours. Control cells were incubated under the same conditions without the addition of nicotine. Cell growth was detected by monotetrazolium salt [3-(4, 5-dimethyl-2-thiazolyl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. Cell apoptosis was detected by Haematoxylin and Eosin staining, immunofluorescence analysis of Filamentous actin and electron microscope observation.
Results: Nicotine had no significant effect on A549 cell growth at the dose of 0.01然 (p>0.05), but had significant growth inhibitory effects at the doses of 0.1然, 1然 and 10然 (p

Key words: Nicotine, A549 cell line, Apoptosis, F-actin



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