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IJMPS. 2014; 4(10): 11-20


STUDIES ON THE EFFECT OF ENVIRONMENTAL DRY STRESS, ACIDITY, ALKALINITY, NaCl, SODIUM HYPOCHLORITE AND LACTIC ACID ON SURVIVAL OF CRONOBACTER SAKAZAKII

Fatimah Y. Abdel-Galil, Hemmat K. Abdel-Latif, Ahmed M. Ammar, Fathy M. E. Serry.

Abstract
Aim: The present study aimed to determine factors that affect survival and growth of C. sakazakii in powdered infant formula milk in order to control the spread of the organism.
Conclusion: C. sakazakii microorganisms resist acid and alkaline effect for more than 30 minutes. At acidic pH 3, 3.5 and 5, there was a decrease in viable counts of C. sakazakii. Also, the same thing at pH 9 and 11, there was decrease in and no detection of the organism at pH 11 for 40 minutes. Using 4% NaCl is an effective mean to kill C. sakazakii in 40 minutes. Sodium Hypochlorite at concentration 4% kills C. sakazakii in 20 minutes. Using lactic acid at concentration 0.3% v/v is considered to be an effective mean to inactivate of C. sakazakii in dehydrated powdered infant formula. Although a majority of C. sakazakii cells were inactivated by storage in dehydrated powdered infant formula, portion of the cells were highly resistant to the storage conditions and survived for extended periods in dehydrated powdered infant formula.

Key words: Cronobacter sakazakii, Powdered Infant Formula milk, survival, dry stress, environmental stress, acidity, alkalinity, lactic acid, sodium hypochlorite, NaCl.



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