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Gulhane Med J. 2012; 54(4): 298-301

The genotype distribution of high risk HPVs in women with suspected cervical lesions

Fatih Şahiner, Kenan Şener, Mehmet Yapar, Murat Dede, Nuri Yiğit, Berksan Şimşek, Ayhan Kubar.

Cervical cancer is associated with Human Papillomavirus (HPV) and is the second most frequently occurring cancer among women and a major cause of cancer mortality worldwide in women. In this study, we used MY09/11 consensus real-time PCR to investigate the presence of HPV-DNA in the cervical smear samples. Secondly, we carried out genotyping analysis using real-time PCR with type-specific primers and probes were designed for the 12 high-risk HPVs (16, 18, 31, 33, 35, 39, 45, 52, 58, 59, 68 and 82). We detected 131 HR-HPV isolates in 106 cervical smear samples that were determined as HPV-DNA positive by consensus PCR. The distribution of
the most frequent high-risk HPV types as follows: HPV-16; 53 (40,5%), HPV-52; 17 (13%) and HPV-58; 14 (10,7%). In addition, according to retrospective data, diagnostic endometrial biopsy was performed in 44 patients (41.5%). The distribution of HPV genotypes in different pathological entities was also assessed. Current HPV vaccines are designed to protect against HPV-16 and HPV-18. More than half (51,9%) of the types identified in this study are except for HPV-16 and HPV-18. HPV-16 and HPV-18 detection rate may be less than this rate, when consider that the screening panel does not contain all HR-HPV types. For these reasons, we think that large-scale studies are needed to determination of the current type distribution in our country to the prediction of protective efficacy of HPV vaccines.

Key words: HPV, genotype, real-time PCR, cervical cancer

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