Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access


The cytokine gene polymorphisms (TNF-α, IL-10 And IFN-γ) and the role of inflammatory cytokines in diabetic neuropathy

M. Ramesh, Konathala Geetha Kumari, G. Sudhakar.

Background: One of the most frequently-occurring micro vascular complications is diabetic neuropathy (DN). Diabetic nephropathy (DN) affects approximately one third of people with type 1 or type 2 diabetes mellitus The objective of the study is an attempt to examine functional SNPs primarily at the position on gene of TNF-α (-308 G/A, rs 1800629), IL-10 (-1082 G/A, rs 1800896) and IFNγ (+874 A/T, rs 62559044) in order to establish their association with peripheral neuropathy patients with type 2 diabetes.
Methods: 150 cases presenting Diabetic neuropathy and 160 cases of age and sex matched healthy controls were included in the study. ARMS PCR was done for genotyping of TNF-α (-308), IL-10 (1082 G/A) and IFNγ (+874) polymorphism using allele specific primers for detection of single nucleotide polymorphisms. Analysis of the data was carried out using Epi Info 5 software. In addition, the gene frequencies were estimated and goodness of fit between the observed and expected phenotype frequencies was tested. Multifactor Dimensionality Reduction (MDR) analysis was performed to study case-control data and gene-gene interactions.
Results: The results revealed that the chi- square test for heterogeneity for IL-10 system was found to be significant (χ = 16.2380; d.f = 2; p >0.001) between patients and controls, indicating a significant departure from the HWE. Thus, the test of association of both homogeneity and heterogeneity of IL-10 showed a significant difference, indicating an association of IL-10 with diabetic neuropathy.SNPs at position −308 promoter gene of TNF-α and IFNγ (+874) were not significantly associated with development of Diabetic Neuropathy.
Conclusion: This case-control study suggests that IL-10-1082G/G polymorphism is associated with the susceptibility to diabetic neuropathy in type 2 DM patients. IL-10 serves as an important bio marker in Indian population for their susceptibility to Diabetic Neuropathy as it may play a role in alteration of IL-10 production and the inflammatory responses.

Key words: Diabetic Neuropathy, Tumor Necrosis Factor Alpha, Interleukin 10, Interferon Gamma

Share this Article

American Journal of Physiology, Biochemistry and Pharmacology


ScopeMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons