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Vet World. 2012; 5(6): 341-345

Filter paper sampling of blood for the detection of antibodies to Infectious Bursal Disease virus using a commercial ELISA kit

Tofayel Ahmed, Md Bashir Uddin, Md Rafiqul Islam, Syed Sayeem Uddin Ahmed, Joyita Basu, Mohammad Mejbah Uddin.

Aim: The present study was designed to investigate the feasibility of the filter paper sampling of blood for the detection of IBDV antibodies in chicken sera using a commercial ELISA kit.

Materials and Methods: Optimum dilution for filter paper extracts that would give a result equivalent to that of the corresponding serum sample in a commercial ELISA was determined. Correlation between the filter paper extracts based ELISA and conventional serum based ELISA was also examined and the reproducibility of the filter paper extracts based ELISA was tested.

Results: A very good correlation (r = 0.9; n = 15; p

Key words: Filter paper extract based ELISA, IBD, Serum based ELISA, Seroprofiling

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