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Vet World. 2012; 5(11): 676-681


Molecular detection of virulence genes associated with pathogenicity of Gram positive isolates obtained from respiratory tract of apparently healthy as well as sick goats

Tushar Kisan Aher, Ashish Roy, Pranay Kumar.

Abstract
Aim: To know the prevalence of gram positive isolates and their virulence genes obtained from respiratory tract of apparently healthy as well as sick goats.

Material and Methods: Nasal swabs and tissue samples were screened for the presence of microbial pathogens by cultural isolation, biochemical confirmation. Further, these isolates were subjected to the virulence gene detection by PCR.

Results: Out of 144 isolates, 57 isolates of gram positive pathogens were obtained and confirmed as Staphylococcus spp. (43 isolates) and Streptococcus spp. (14 isolates) on the basis of Gram staining, morphology, cultural identification and biochemical characters. Five isolates (11.6%) were found to be positive for Coagulase gene; 11 isolates (25.6%) were found to be positive for clfA gene; and 14 isolates (32.6%) were found to be positive for Spa gene.

Conclusion: The presence of these genes confirmed the pathogenic potential of gram positive pathogens and their association with clinical manifestations in respiratory tract infections of goats.

Key words: biochemical characters, PCR, respiratory tract of Goat, Staphylococcus, Streptococcus, virulence genes



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