Water in oil emulsion fowl cholera vaccine was prepared using Montanide ISA-70-VG oil as alternative adjuvant to the used white mineral oil with span-80. The two vaccines were comparatively evaluated in SPF chickens. Each vaccine was inoculated in a group of 40 chickens while the third group of 40 chickens was kept as non-vaccinated control. It was noticed that the Montanide ISA-70-VG formulated vaccine induced mild transient local inflammation in comparison with those induced by the mineral oil formulated vaccine. Challenge test and application of indirect haemagglutination test on serum samples obtained from all chicken groups revealed that Montanide ISA-70-VG formulated vaccine induced earlier and higher immune response than that induced by the mineral oil formulated vaccine. So it could be recommended to use Montanide ISA-70-VG as adjuvant to fowl cholera vaccine aiming to provide early and high immune response able to protect chickens against fowl cholera infection.
Pasteurella multocida, chicken, vaccination, isa70, whiterex oil.