In vitro antifungal susceptibility testing is important to guide the clinicians to choose the correct antifungal for patients. Broth microdilution method (BMD) recommended by CLSI is the reference method for antifungal susceptibility testing of filamentous fungi. However, the method is time consuming and labour intensive and not practical for clinical laboratories. Therefore alternate approaches are in demand. E test is surface agar based quantitative MIC determination method. We evaluated ESYMIC (Fluconazole) and HICOMB MIC (Ketoconazole) manufactured by HIMEDIA, Mumbai for MIC determination against 60 isolates of Trichophyton species against BMD. MIC ranges for T. rubrum, T. mentagrophyte and T. tonsurans for Fluconazole were 4-256, 1-64 and 0.5-2 respectively and MIC range of 0.25-2 for Ketoconazole for three species. Determination of MIC by E test seems to be easy alternative to BMD for Fluconazole and Ketoconazole. MICs noted for Ketoconazole by E test correlated well with BMD method but not for Fluconazole.
antifungal susceptibility, E test, MICs, Dermatophytoses, Trichophyton,