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Emir. J. Food Agric. 2011; 23(4): 320-329


Molecular diagnosis of Trypanosoma evansi infection in
Camelus dromedarius from Eastern and
Western regions of the Sudan

Nahla O. M. Ali1
?, Hamid I. M. N. Croof2
and Hamid S. Abdalla1.

Abstract
Trypanosoma evansi infection is the most important disease of Camel in the Sudan. Thepresent work was carried out to evaluate a simple PCR-based technique for field diagnosis of T.evansi infection in camels from Eastern and Western regions of the Sudan. A representativenumber of 600 camels (Camelus dromedarius) from different areas of Gedarrif State (Eastern) andNorth Kordofan State (Western) were examined from May 2005 to July 2007 for Trypanosomaevansi infection. The tests used were parasitological (Wet Smear Film, WSF; Thin Smear Film,TSF; Buffy Coat, BC), serological (Card Agglutination Test/T. evansi, CATT), and DNAamplification by polymerase chain reaction (PCR). The prevalence of T. evansi infection in camelswas detected in 36 (out of 40), 100 (out of 210), 36, 22, 10 (Out of 600); by PCR, CATT, TSF, BCand WSF with sensitivity of 90%, 47.6%, 6%, 3.7% and 1.7%, respectively. PCR revealed aspecific 200 bp band in positive samples. The intensity of PCR bands was variable in different testsamples depending upon the level of infection in the test samples. The history of intermittentfever, emaciation, oedema, poor body condition significantly correlated with positive serologicalstatus in CATT as well as trypanosome DNA detection by PCR. As there are no previous studiesin the Sudan on the molecular characterization of the parasite, this research is useful informulating strategic control programmes.

Key words: Trypanosoma evansi, camels, molecular diagnosis, Sudan



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