Tomato Yellow Leaf Curl virus (TYLCV-Eg) was isolated from whiteflies-infectedtomato (Lycopersicon esculentum cv.Castle rock) plants growing in Nubaria and El-BeheraGovernorate. The infected plants exhibited systemic viral symptoms in the form of sever leafcurling, leaf crinkle with marginal yellowing, stem upright, twisted and stunted. TYLCV-Egreacted positively with polyclonal antibodies specific to TYLCV using DAS-ELISA. It wastransmitted by both syringe injection and whiteflies with transmission efficiency of about 80% and100%, respectively. TYLCV-Eg isolate was transmitted to different species belonging to familiesCucurbitaceae, Fabaceae, Solanaceae and Chenopodiaceae. TYLCV had TIP of 70ºC, DEP of10-7 and LIV of about 6 days. Electron micrograph of the partially purified TYLCV revealed thepresence of monomer and dimmer gemini particles with dimensions of 22 nm and 20 × 30 nm to24 × 30 nm, respectively when negatively stained with uranyl acetate. Using degenerateoligonucleotide primers, the viral coat protein gene was amplified successfully by PCR, producing~ 500 bp fragment from tomato infected plants. The viral genome was detected by specific DNAprobe using dot blot hybridization technique. Comparative nucleotide sequence analysis showed asimilarity of 98% between TYLCV-Eg and other isolates.
TYLCV-EG, DAS-ELISA, PCR, Dot-blot hybridization, and Nucleotide sequence