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32

Emir. J. Food Agric. 2012; 24(5): 425-433


A new concept for production and scaling up of bioactive compounds from
Egyptian date palm (Zaghlool) cultivar using bioreactor

H. S. Taha*
, S. A. Bekheet and M. K. El-Bahr.

Abstract
A promising and successful protocol for enhancement and production of total phenolic and peroxidescompounds from Egyptian date palm cultivar Zaghlool cells in stirred tank reactor was established. Theinfluence of cell culture cultivation in combination with Aspergillus niger extract, and methyl-jasmonateelicitors incorporation in the feeding medium on cell growth patterns and production of active compounds wasinvestigated. The maximum value of cell growth parameters and the highest content of bioactive compoundswere obtained from elicitation of modified MS-medium with Aspergillus niger extract at 0.1% in combinationwith methyl-jasmonate (100 M), as compared with other concentrations after 10 days of cultivation. Thechemical analyses of phenolic and peroxidase compounds were spectrophotometrically performed.

Key words: Date palm, Elicitors, Phenolic and Peroxidase compounds, Bioreactor



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