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Egypt. J. Exp. Biol. (Bot.). 2013; 9(1): 153-158


EVIDENCE FOR GENOTYPING SIMILARITY BETWEEN HELICOBACTER PYLORI STRAINS ISOLATED FROM DENTAL PLAQUE AND PEPTIC ULCER

El-Baghdady K. Zakaria Abd El-Ghany M. Gad Abdel-Ghafar H. Abdel Aziz Abou-Zeid, M. Abd El-Montaser Abd El-Salam M. Mahmoud.

Abstract
In this study, genotypes of Helicobacter pylori (H. pylori) strains isolated from dental plaque specimens and peptic ulcer biopsies of the same patient were compared. Gastric biopsies of 30 patients out of 64 cases suffering from gastric ulcer (46.88%) were H. pylori positive. H. pylori was isolated from 4 dental plaque specimens (6.65%) of the number of patients examined. Amplification of UreC gene confirmed that the 34 isolates were H. pylori. All isolates of H. pylori were phenotypically identical. Partial sequence of 16S rRNA gene for H. pylori isolates from dental plaque (HK1D and HK2D) and peptic ulcer biopsies (HK1B and HK2B) of two patients were identical with 99% to H. pylori B38 reference isolate. A single nucleotide substitution (T instead of C) was detected. Whereas, nucleotide sequence analysis of H. pylori isolates from a third patient (HK3B, HK3D) was 100% identical to H. pylori G27 reference strain.

Key words: Helicobacter pylori, UreC, dental, gastric



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