In the present study two genera of green macroalgae, Spirogyra sp. and Chara sp. were collected from freshwater of Beastan swr spring in October 2011. The physico-Chemical parameters of water measured were temperature, pH, conductivity, turbidity, alkalinity, nitrate, phosphate, and sulphate. Oil was extracted from dried algae samples using hexane and petroleum ether as solvents. The percentages of oil extracted were 3.75% and 1.06% from Chara sp., respectively, while 0.69% and 4.24%of oil were extracted from Spirogyra sp., respectively. The most abundant fatty acids were arachidic acid, erucic acid, and docosadienoic acid which determined by HPLC –Technique .The ratio of arachidic acid C20: 4, erucic C22: 1, and docosadienoic C: 22:2 were recorded as 66.03 µg/ml, 94.36 µg/ml and 93.45 µg/ml, respectively from Chara sp. while 89.97 µg/ml of arachidic acid 190.86 µg/ml of erucic acid and 101.28 µg/ml of docosadienoic acid from Spirogyra sp.
Spirogyra, Chara, Fatty acid, Physico-chemical parameters, Oil extraction