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Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 207-214


MULTI-DRUG RESISTANCE AND SLIME PRODUCTION IN COAGULASE NEGATIVE STAPHYLOCOCCI IMPLICATED IN NOSOCOMIAL INFECTIONS IN MANSOURA HOSPITALS, MANSOURA, EGYPT

Fathy A. Mansour *Mahmoud M. Zaky **Tarek H. El-Morsy.

Abstract
Coagulase negative Staphylococci (CoNS) are known as commensals organisms of the human skin and mucous membrane. During this study clinically significant CoNS accounted for 43, 40, 25, 20, 12, and 11 % of pathogen were isolated from blood, urine, throat, fluid from drain, wound and ear, respectively. Staphylococcus epidermidis was the far most frequently isolated species 87% of the population studied followed by S. saprophyticus 13%. Slime production was detected using Congo red, adherence tube and spectrophotometric methods. It was found that, the best carbohydrate source is glucose and the organic nitrogen source is mixture of casein and soy bean meal for slime production. Serum did not support slime production as NaCl does. All strains were tested for their antimicrobial susceptibility using diffusion disc method. Strains showed multiple antibiotic resistance to amoxicillin, oxacillin, tetracycline and penicillin G, and less resistant to cefuroxime, imipenem, erythromycin, trimethoprim + sulphamethazole and pipracillin, on the other hand, they were sensitive to vancomycin and ciprofloxacin.

Key words: Multi-Drug resistant, Nosocomial infections, Coagulase negative Staphylococci (CoNS) Slime production.



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