Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research

Egypt. J. Exp. Biol. (Zoo.). 2005; 1(0): 65-72


PROTEIN AND ISOZYMES ELECTROPHORESIS FOR IDENTIFYING SOME CARP SPECIES

Hussain A. Elghobashy *Mohamed A. Rashed *Salwa M. Mohamed Ashraf A. Ramadan.

Abstract
Genetics similarity and diversity of five cultured carp fishes collected from Abbassa area (CLAR) in Sharkia, Egypt, were examined. SDS-PAGE for muscle protein and native-PAGE for some isozymes, such as esterases and malate dehydrogenase were studied on ten fish specimens of each species. To discriminate isozymes variation among carp species, different synthetic esterases substrates; α, β-naphthyl acetate and α- naphthyl propionate were tested. The results indicated that the soluble muscle protein patterns it may be conducted that each population had a unique banding pattern. Close relationship was noticed between bighead and black carp with similarity coefficient value of (94%). Silver carp had a different banding pattern and branched out as subgroup from each of bighead and black. The results of α, β naphthyl esterase showed perfect similarity within silver individuals. The average similarity values among the five carp species resulted from cluster analysis of α, β esterase isozymes data showed a close relationship between grass and silver carp (95%). High similarity in black, mirror carp, and grass carp while bighead and silver carp showed rear polymorphism with propionate esterase. Also the result showed that the variation in malate dehydrogenase was limited and restricted on silver and mirror carp, while bighead, black and grass carp showed no variation within their populations. The final data of this work showed close relationship between bighead and black carp (85%). Moreover, quite close relationship was also noticed between each of black and silver carp followed by bighead and silver carp.

Key words: Electrophoresis, identification, protein, isozymes, carp species.



Share this Article


Advertisement
Applied Medical Research

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons