Isosorbide dinitrate "ISDN" is an organic nitrate that generates nitric oxide "NO" that helps in treatment of the vascular disturbance which results from metabolic hypercholesterolemia. Forty two adult male albino rats weighting 170-190 g were divided randomly into six groups, each of seven rats, as follows: control group (standard diet), coconut oil treated group (0.5 ml daily), cholesterol treated group (400 mg/kg BW dissolved in 0.5 ml coconut oil), ISDN treated group (1.0 mg/kg BW dissolved in 0.5 ml H2O), ISDN plus cholesterol treated group (ISDN + cholesterol) for 3 weeks and cholesterol followed with ISDN treated group (cholesterol for 3 weeks then ISDN for 3 weeks) by oral administration. Serum nitric oxide, antioxidant enzymes "superoxide dismutase (SOD) and glutathione peroxidase (GPx)" activities, lipid profile, glucose, uric acid, total protein, albumin and globulin percentage were determined. At the end of the experimental period, coconut oil treated group showed a significant increase in serum of both high density lipoprotein "HDL" and very low density lipoprotein "VLDL" and a significant decrease in serum SOD activity as compared to the control group. Cholesterol treated group showed a significant increase in total cholesterol "TC" and low density lipoprotein "LDL-C" and a significant decrease in NO level, SOD and GPx activity as compared to the control one. ISDN administration showed a significant increase in the triglyceride "TG", VLDL, and NO levels, but exhibited a significant decrease in LDL, albumin and GPx activity compared to control group. The ISDN plus cholesterol group showed a significant increase in LDL-C level and a significant decrease in albumin and GPx activity compared to the control one. The group treated with cholesterol followed with ISDN showed significant increase and decrease in HDL level and SOD activity, respectively compared to the control group. ISDN administration after cholesterol induction helped in the return of most studied parameters to the control values.
Hypercholesterolemia, Isosorbide dinirate and Nitric oxide