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J App Pharm Sci. 2015; 5(Supplement 2): 091-098


Trichoderma asperellum isolated from salinity soil using rice straw waste as biocontrol agent for cowpea plant pathogens

Eman R. Hamed, Hassan M. Awad, Eman A. Ghazi, Nadia G. El-Gamal, Heba S. Shehata.

Abstract
This study discusses isolation and identification new fungus isolate from salinity soil for controlling soil borne diseases. Among sixteen fungal, a potent isolate coded SRBP_ZSHSG1 was isolated from Sugar beet rhizospher samples collected from Al-Hosainia localities- El-Sharkia- Egypt. Traditional methods consistent with phylogenetic analysis of 18S rRNA sequences showed SRBP_ZSHSG1 has 100% similarity with Trichoderma strains and the most closest is Trichoderma asperellum. Thus, it proposed name Trichoderma asperellum SRBP_ZSHSG1 (ID: KP336489). Results proved SRBP_ZSHSG1 followed by T. roseum and Chaetomium globosum were highly inhibitors to the tested pathogens. These results were confirmed by field experiments. SRBP_ZSHSG1 was able to grow on rice straw (biostraw) and produce most active compounds. The biostaw extract was the most effective bioagent and recorded highest reduction in pathogen numbers. GC/MS analysis of ethyl acetate extract revealed the presence of 9 compounds. These compounds were determined 4 volatile alcohols (1-4) and fatty acid esters (5-9).

Key words: Trichoderma sp., phenotypic and genotypic identification, Root rot, Wilt, cowpea plant.



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