Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article

J App Pharm Sci. 2015; 5(11): 023-030


Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.

Mariquit M. De Los Reyes, Glenn G. Oyong, Virgilio D. Ebajo Jr., Vincent Antonio S. Ng, Chien-Chang Shen, Consolacion Y. Ragasa.

Abstract
The triterpenes, squalene (1), friedelin (2) and a mixture of ursolic acid (3a) and oleanolic acid (3b) in a 2:3 ratio, and a mixture of ╬▓-sitosterol (4a) and stigmasterol (4b) in a 2:1 ratio, obtained from the dichloromethane extract of Pipturus arborescens (Link) C.B. Rob., were evaluated for their anti-proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast- neonatal (HDFn) using the in vitro PrestoBlue« cell viability assay. The HCT-116 cell line was most susceptible to the compounds and mixtures tested. Triterpene 1 was most cytotoxic against HCT-116 and MCF-7 with IC50 values of 4.21 and 5.92 ╬╝g/mL, respectively. Triterpene 2 and the mixture of 3a and 3b were highly anti-proliferative against HCT-116 cells (IC50 of 1.22 and 1.66 ╬╝g/mL, respectively) and moderately inhibitory against MCF-7 cells (IC50 of 16.51 and 23.97 ╬╝g/mL, respectively). The mixture of 4a and 4b exhibited high cytotoxicity against HCT-116 cells (IC50 of 1.14 ╬╝g/mL). Compounds 1-4b showed the least activity against HT-29 cells (IC50 of 11.97 to 52.52 ╬╝g/mL). Cytotoxic effect was not observed against HDFn cells (>100 ╬╝g/mL). Comparing the effects of 1-4b on the two colon cancer cell lines, the IC50 values of 1-4b against HCT-116 were lower than those of HT-29.

Key words: Pipturus arborescens (Link) C.B. Rob., squalene, friedelin, ursolic acid, oleanolic acid, ╬▓-sitosterol, stigmasterol, cytotoxicity, MCF-7, HCT-116, HT-29, HDFn



Share this Article


Advertisement
Journal of Apitherapy

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ę ScopeMed« Information Services.
Scopemed Buttons