Introduction: Pseudomonas aeruginosa is an important nosocomial pathogen characterized by its innate resistance to multiple antimicrobial agents. Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism.
Aims and objectives: Aim of the present study is to detect slime production in clinical isolates of Pseudomonas aeruginosa by two phenotypic methods as tube method and spectrophotometric method.
Material and method: Total isolates were identified by standard microbiological procedure and subjected to antimicrobial susceptibility testing as per CLSI guidelines. Slime production in all isolates was detected by using Tube method and Spectrophotometric method.
Results: Out of total 100 isolates of Pseudomonas aeruginosa 59% were slime positive by Tube method and 34% by Spectrophotometric method. Tube test was found to be superior test as compared to the Spectrophotometric method.
Conclusion: Tube method is more qualitative and superior method to detect slime producing P. aeruginosa isolates.
Pseudomonas, slime production, Virulence, Biofilm.