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IJHSR. 2015; 5(10): 90-100

Identification of Species, Their Prevalence and Antimicrobial Susceptibility of Enterococci Isolated From Urine Samples in a Tertiary Care Hospital in Bengaluru.

Smeeta Huidrom, Girish N.

Background: Enterococci, though considered as normal commensal of the intestinal tract, have emerged as medically important nosocomial pathogens. The emergence of Vancomycin Resistant Enterococci (VRE) in addition to the increasing incidence of High Level Aminoglycoside Resistance (HLAR), presents a serious challenge for clinicians.
Materials and Methods: This is a hospital based prospective study carried out in the Department of Microbiology, VIMS & RC, Bengaluru, during a period of one year from Jan 2013 to Dec 2013. Identification and speciation of the isolates were done by the standard conventional methods and antibiotic sensitivity pattern was determined according to CLSI guidelines. All isolates suspicious of resistance to Vancomycin by disc diffusion method were further put up for E-test to determine their Minimum Inhibitory Concentration (MIC).
Results: A total of 105 strains of Enterococci were isolated, of which 102 (97.2%) were Enterococcus faecalis, 02 (01.9%) were E. faecium, and 01 (00.9%) was E. dispar. Enterococcal isolates showed good sensitivity to Linezolid and Nitrofurantoin. A total of 33 isolates showed high level resistance to Gentamicin and 39 to Streptomycin by high content disc diffusion. Vancomycin resistance (MIC ≥ 32 μg/ml) was seen in 4 (3.8%) isolates.
Conclusion: Among the enterococcal isolates from urine samples E. faecalis was the commonest. Antibiotic sensitivity pattern revealed presence of multidrug resistance in E. faecalis and also in E. faecium, HLAR and Vancomycin resistance. Disparity in detection of VRE by disc diffusion method was observed when compared with the E-test, highlighting the importance of accurate determination of MIC for Vancomycin.

Key words: Enterococcus species, HLAR, VRE, MIC.

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