Background: Secondary alveolar bone grafting of the cleft alveolar ridge in the mixed dentition is a well-established treatment
for patients with cleft lip and palate (CLP). Recently tissue engineering has become available as a regenerative treatment for
bone defects; however little has been reported on application of tissue engineering for regeneration of cleft defect tissues. Mesenchymal
derived stem cells were applied to different kinds of bone substitute in attempt to overcome the drawbacks of bone
Materials and methods: Eight patients with alveolar cleft were included in this study; the cleft repair was done using bone marrow
derived stem cell loaded on NanoBone® scaffold. All patients were followed up clinically and radiographically for 6 months
Results: The successful healing was observed without oronasal fistula. In all patients the closure of the alveolar arch and
stabilization of the teeth adjacent to the cleft were achieved. The radio-opacity in serial computed tomography slicing the middle
level of the alveolar cleft in the grafted region increased gradually over the time.
Conclusion: Tissue engineered bone from mesenchymal stem cells may provide an acceptable alternative especially in the
case of limited autograft availability or donor site morbidity.
Alveolar cleft, Stem cell, Tissue engineering