Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

1

IJAVMS. 2010; 4(2): 47-55


Media Optimization for Hyper-production of Carboxymethyl Cellulase using proximally analyzed agro-industrial residue with Trichoderma harzianum under SSF

Hafiz Muhammad Nasir Iqbal, Muhammad Asgher, Ishtiaq Ahmed, Shahbaz Hussain.

Abstract
Now a day’s enzyme production is a growing field of biotechnology especially related to industrially important enzymes. The present study was carried out to investigate the potential of a filamentous fungus, Trichoderma harzianum for hyper-production of third most demanded industrial enzyme carboxymethyl cellulase using cheap and easily available agro-industrial residue wheat straw as growth supporting substrate under still culture solid state fermentation technique. Production of carboxymethyl cellulase was substantially enhanced through media optimization process. To promote carboxymethyl cellulase production, we evaluated the effect of several kinetic parameters like pretreatment, substrate concentration, initial moisture content, pH, incubation temperature and inoculum size on carboxymethyl cellulase production. Samples were harvested after every 24 hrs to study the profile of cellulase enzyme produced by the fungus on proximally analyzed wheat straw. By optimizing the SSF medium containing 2 % HCl pretreated wheat straw; maximum carboxymethyl cellulase activity (4804.22 M /mL/min) was recorded after 7th day of incubation at pH 5.5; temperature, 35C; moisture, 40 % and inoculum size, 10 %, using optimum substrate concentration (3%). Outcome of the research will be supportive in the improvement of low cost system for hyper-production of carboxymethyl cellulose for industrial application.

Key words: Carboxymethyl cellulase, lignocellulosic residue, media optimization, proximal analysis, SSF, Trichoderma harzianum



Share this Article


Advertisement
Journal of Apitherapy

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons