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J Health Sci KOU. 2016; 2(1): 20-22


RE-EVALUATION OF LPS CONCENTRATIONS ON THE 293T HUMAN RENAL CELL LINE

Aris Cakiris, Atilla Cakar, Neslihan Abaci, Sema S. Ekmekci, Zeliha Emrence, Duran Ustek.

Abstract
Objective: The inflammatory responsiveness of the cells to Lipopolysaccharides (LPS) is commonly used for in vitro experiments. However, the correct dose of the LPS for cell line experiments is elusive. The LPS concentration that gives the maximal response is a critical point of in vitro inflammatory experiments. The aim of this study was to reevaluate the concentration dependent effect of LPS on the 293T human renal cell line.
Methods:We evaluated the cell-detachment assay of LPS-stimulated 293T cell line monitored by xCELLigence Real-Time Cell Analyzer (RTCA) system. We applied increasing concentration of LPS followed by Roche xCELLigence Instrument based on the Real-Time Cell Analysis System.
Results:Our results demonstrated that the 2, 4 and 8μg of LPS inhibit cell division which diverts cells to a steady-state phase, 1 μg acts as mitogen. Lower concentrations are no effect on cells.
Conclusion:These work showed that LPS concentrations had various effects on cell proliferation and need to be estimated for each experiment before carry out the experiments.

Key words: Lipopolysaccharides(LPS), cell Proliferation, inflammation



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