Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access


IJAVMS. 2008; 2(0): 14-17

Prevalence of Genotype 3a Hepatitis C Virus (HCV) In the Infected Population of Lahore, Pakistan

Tayyaba Ijaz, Muhammad Khurram Shahzad, Noeen Sarfraz, Muhammad Athar Khan.

Hepatitis C virus (HCV) is most important public health concern and an emerging disease in
Pakistan. A study was conducted to explore the prevalence of Hepatitis C.Genotype 3a in the infected
population of Metropolitan, Lahore Pakistan by using primers against the 5\' non coding region of the viral
RNA, cDNA was synthesized. For qualitative analysis, the amplification of cDNA was done by first round
PCR. Nested PCR was performed with first round PCR product. Amplification of HCV cDNA for
genotyping by regular PCR was carried out. For HCV genotyping by second round PCR, two different
primer mixtures were prepared. Detection of genotype specific band was confirmed by gel electrophoresis.
A 232 bp specific band of HCV 3a genotype was amplified. Our result showed, prevalence of genotype 3a
out of 155 HCV PCR positive samples where 51.61 % (80) samples were among population of Lahore.

Key words: 3a, Genotype, HCV PCR positive samples.

Share this Article

Journal of Behavioral Health


ScopeMed Home
Follow ScopeMed on Twitter
Article Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons