Isolation of pathogenic Escherichia coli from buffalo meat sold in Parbhani city, Maharashtra, IndiaC. S. Shekh, V. V. Deshmukh, R. N. Waghamare, N. M. Markandeya, M. S. Vaidya.
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Aim: Isolation, characterization, in-vitro pathogenicity and antibiogram study of E.coli from buffalo meat sold in Parbhani city.
Materials and Methods: Meat samples were collected from buffalo immediately after slaughter. Isolation, identification and enumeration of E. coli were done by following standard methods and protocols. Hemolysin test and Congo red binding assay were used to study in-vitro pathogenicity of E. coli isolates. Disc diffusion method was used to study antibiogram of pathogenic E. coli isolates.
Results: A total of 250 buffalo meat samples were collected and processed. A total of 22 (8.80 percent) E. coli isolates were isolated with average differential count of 1.231 ± 0.136 log cfu/g on EMB agar. All the E. coli isolates were confirmed by 10 Grams staining, biochemical reactions and sugar fermentation and motility tests. A total of 9 (3.6 percent) E. coli isolates were found to be pathogenic by in-vitro pathogenicity testing. Antibiogram studies of pathogenic E. coli isolates showed that all 9 isolates were sensitive to gentamycin (20 ± 1.49 mm) while 7 isolate showed resistance to enrofloxacin (18.22 ± 3.58 mm) and tetracycline (11.44 ± 2.04 mm).
Conclusion: Buffalo meat sold in Parbhani city is an important source of E. coli infection to human population. A total of 9 pathogenic E. coli were isolated from buffalo meat immediately after slaughter. All isolates were characterized and confirmed pathogenic by in-vitro pathogenicity tests. Antibiogram studies of all isolates revealed sensitivity to gentamicin and resistance to tetracycline and enrofloxacin.
antibiogram, buffalo meat, E. coli, pathogenicity