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Egypt. J. Exp. Biol. (Zoo.). 2016; 12(1): 30-49


DETECTION OF EGYPTIAN BANCROFTIAN FILARIASIS IN SUSPECTED PATIENTS SUFFERING LYMPHOEDEMA USING SEROLOGICAL AND PCR TECHNIQUE

Alaa Abd El-Aziz Mohammad Samn.

Abstract
This study was conducted at Jan, 2014 to November, 2015 on a total of 54 patients suspect to be infected by filariasis due to their clinical picture (suffering from lymphoedema) and another 20 non lymphoedematous cases. All living in endemic areas. Patients were attending tropical, internal medicine, vascular surgery and pediatric departments of the following hospitals: Sherbien, Dakahlia (4 cases), El-Saf, Giza (17 cases), Kafr Saker and Fakous, Belbes central hospital, Sharkia (23 cases), Shebien, Menofia (4cases) and Al-Hussien-Cairo (6 cases) hospitals. Prevelances of infected Patients were 31 females (57.4%) and 23 males (42.6%) in ratio 1:1.3 respectively. Cases were divided into 4 groups according to their ages and oedema incidence. For detection of microfilaraemia, three methods were employed namely thick blood film, knott’s technique and nucleopore filtration technique. As regard to microfilaria, there were 30 cases (55.56%) negative and 24 cases (44.44%) positive. In patients group 29 negative cases (53.70%) for IgM while 25 positive cases (46.30%) for IgM. In patient groups, 29 negative cases (53.70%) for IgG and 25 cases (46.30%) were positive. Results of PCR, revealed 18 positive cases (33.3%) of the studied group and 36 negative cases (66.7%) for PCR.

Key words: Bancroftian Filariasis, Lymphoedema, Serological (Ag-Ab) And PCR Technique



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