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. 2016; 3(1): 20-27


Comparative study between direct and indirect isolation of Listeria monocytogenes and its antimicrobial resistance

Yousef* M, Adel AEK, Samir AEG, Maha AA.

Abstract
In the present study, Listeria monocytogenes was detected by using direct and enrichment methods and comparing between the two methods in isolation. A total of 200 samples (50) of each raw market milk, individual farm milk and Kariesh cheese plus (25) of each Talaga cheese and Damietta cheese) were collected randomly from dairy farms, different shops and supermarkets in Mansoura city, Dakahlia, Egypt. It declared that L. monocytogenes by direct platting method was detected 10%, 8%, 8% and 4% in raw market milk, Kariesh cheese (acid curd soft cheese), Talaga (fresh soft cheese) and Damietta cheese (salted cheese), respectively while farm milk was free from L. monocytogenes. Also L. monocytogenes, in milk and white soft cheese by enrichment method (indirect) was 8% and 4% in raw market milk and Talaga cheese respectively. L. monocytogenes wasn’t isolated from farm milk, Kariesh cheese and Damietta cheese. Antimicrobial susceptibility test was done to L. monocytogenes where 100% isolates were resistance against Oxytetracycline (T) followed by Penicillin (P) 96.5%, Neomycin (N) 93.1%, Nalidixic acid (NA) 89.7%, Kanamycin (K) 82.8%, Chloramphenicol (C) 75.9%, Erythromycin (E) 72.4%, Amoxicillin (AMX) 68.9% Streptomycin (S) 58.6%, Sulphamethoxazol (SXT) 55.2%, Ciprofloxacin (CP) 44.8%, Ampicillin (AM) 31%, Gentamycin (GM) 13.8% and Norfloxacin (NOR) 10.3%.

Key words: Listeria monocytogenes, isolation, antimicrobial resistance



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