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Egypt. J. Exp. Biol. (Bot.). 2016; 12(1): 145-154


MONITORING IMPORTED GRAIN-BASED INGREDIENTS USED IN FEED PROCESSING FOR TOXIGENIC MOULDS AND NATURALLY OCCURRING MYCOTOXINS

Dalia A. M. Abdou, Rasha M. Othman, Nasr. E. El-Bordeny, Nevin A. Ibrahim Mohamed A. Abouzeid.

Abstract
Samples of imported feeds and feed ingredients were collected every three months during the study time from feed factories in two governorates, Egypt. Samples were cultured and the recovered isolates of Fusarium and Aspergillus were mycologically identified using the universal keys. Species of Fusarium were the prevalent with relative density of 52.9%. The majority of samples analyzed (67.7%) had moderate fungal counts (around 8.9 103 CFU g-1) whereas 26.2% contained counts over the feed hygienic quality limits of 1104 CFU g−1. Based on frequency in tested feed samples as well as relative density, F. proliferatum was the predominant Fusarium species (23.8%) whereas 36.9% yielded isolates of A. flavus. Applying high performance liquid chromatography technique, Applying chromatographic techniques, T-2 toxin (0.0493 mmol/L) was detected in the culture filtrate of a single isolate of F. sporotrichioides whereas two different isolates of F. verticillioides and an isolate of F. proliferatum produced fumonisin B1 in amounts of 0.094 mmol/L, 0.1530 mmol/L, and 0.212 mmol/L. Two A. flavus isolates were capable of producing aflatoxin B1 (0.0580 mmol/L and 0.0446 mmol/L) and aflatoxin B2 (0.0202 mmol/L and 0.0236 mmol/L). Aflatoxins G1 (0.0744 mmol/L) and G2 (0.0433 mmol/L) were produced in the culture filtrate of a single isolate of A. parasiticus. Only three feed samples were naturally contaminated with traces of mycotoxins, a sample of Argentinean corn which contained T-2 toxin in addition to two locally produced samples of broiler finisher feed which contained traces of fumonisin B1 and dairy cattle CFM which contained aflatoxin B2 and a trace amount of aflatoxin B1.

Key words: Feed Stuffs, Toxigenic Moulds, Grain's Contamination Level, Fusarium, Aspergillus mycotoxins.



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