BACKGROUND/OBJECTIVES: Majority of urinary tract infection (UTI) patients received at
Liaquat University Hospital Jamshoro and Hyderabad belong to adjoining rural areas of
Sindh province. As only reliable clinical laboratory test available for diagnostic purpose is
urine culture and sensitivity which is lacking in these areas due to complexity of tests, expertise
and non-availability of electric facility. Hence, present study was conducted to device
alternate method, which must be simple, easy and does not require much expertise.
PATIENTS AND METHODS: In all, 106 patients and 25 normal controls were selected for this
study. Morning urine samples were collected and analyzed for amino acids by paper chromatography
using two different solvent systems.
RESULTS: Different amino acids were found missing in urine of UTI patients. Greater percent
of them was aromatic amino acids like tryptophan (90.56%), phenylalanine (71.69%), tyrosine
(70.75%) and methionine (91.5%).
CONCLUSION: Ordinary urine chromatographic separation test of amino acids shows aromatic
and essential amino acids absent from urine, which can provide basis for screening test
of UTI in areas with lesser facilities.
Amino acids. Urinary tract infection. Chromatography. Aromatic amino acids.
Essential amino acids.