The aim of this study was to investigate the possible contribution of the anti-inflammatory and antioxidant effects of D-004, the lipid extract of the royal palm (Roystonea regia) fruits, on testosterone-induced prostate hyperplasia (PH) in rats. Rats were randomized into seven groups: a negative control and six groups were injected subcutaneously with testosterone; one of the testosterone-injected groups served as positive control and five were treated with D-004 (400 mg/kg), grape seed extract (GSE) (250 mg/kg), vitamin E (VE) (250 mg/kg), ibuprofen (200 mg/kg) or celecoxib (50 mg/kg) for 14 days. Effects on prostate weight (PW), on rat prostate malondialdehyde (MDA) concentrations and myeloperoxidase (MPO) activity were assessed. The injection of testosterone increased significantly the PW, PW/bodyweight (BW) ratio, MDA concentration and MPO activity in the positive controls as compared to the negative controls. Oral administration of D-004, not of any other treatment, significantly reduced PW and PW/BW ratio as compared to the positive control. D-004, GSE and VE, not ibuprofen or celecoxib, lowered significantly MDA values (82.9, 86 and 98.8%, respectively). In contrast, significant inhibitions of MPO activity were achieved with D-004 (58.5%), GSE (56.1%), ibuprofen (73.8%) and celecoxib (61.5%), not with VE. In conclusion, oral treatment with D-004 (400 mg/kg), but not with the antioxidant and anti-inflammatory substances tested, prevented testosterone-induced PH in rats, so that the preventive effect of D-004 on this model does not seem to be associated to its antioxidant or anti-inflammatory effects.
Anti-inflammatory; D-004; Prostate hyperplasia; Rat