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Natl J Integr Res Med. 2013; 4(1): 38-44

Variability And Accuracy Of Sahliís Method In Estimation Of Haemoglobin Concentration

Dr Prashant Jijabrao Patil*, Dr Girish Vithal Thakare**, Dr Sarika Prashant Patil***.

Background: Haemoglobin concentration provides information about the status of anaemia in thepopulation. Haemoglobin estimation is the routine and frequently performed screening haematological test of laboratory services. The Type of methods used and sites of collection of blood samples has found to make differences in haemoglobin concentration. The methods used in routine measurement are needed to be adjusted to obtain comparability with the haemiglobincyanide method. So the present study was undertaken with a objective to test the reliability of Sahliís method in estimation of haemoglobin concentration and standardizing it in district hospital laboratory of Dhule in Maharashtra. Material & Methodology: Haemoglobin concentration (gm/dl) in blood is compared in 173 apparently healthy medical college students within 18 to 23 years age. Results from Sahliís method were compared to Haemiglobincyanide (HiCN) method within capillary and venous blood of same subjects. Results: Sahliís method has lower values than Haemiglobincyanide method with mean difference of 0.62gms/dl(95%CI; 0.51 to 0.73, p

Key words: Haemoglobin, Sahliís Method, Haemiglobincyanide Method

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