Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

1

JARMS. 2013; 5(1): 19-22


Rapid detection of Rifampicin resistance in sputum samples using indigenously developed molecular probes and comparison with conventional MIC Method.

*Manoj Kumar Meghwani,***Shrawan Kumar,**Ram Das,*Rajesh Kumar Gupta.

Abstract
ObjectiveTo assess the efficacy of indigenously developed molecular probes in detection of rifampicin resistance in Mycobacterium tuberculosis directly in sputum samples and comparison with rifampicin susceptibility by conventional MIC method at 64 mcg/ml.
Material and MethodsA total of 81 sputum samples from patients of suspected drug resistant sputum positive for AFB pulmonary tuberculosis were included. All the samples were tested for rifampicin resistance both by MIC method at 64 mcg/ml in LJ media and by indigenously developed molecular probe method simultaneously. ObservationOut of 81 samples,culture on LJ media showed that 48.15% were culture positive while 43.21%were culture negative and 8.64% were contaminated. Rifampicin sensitivity profiles of 28 Mycobacterium tuberulosis isolates with conventional method on LJ media showed that 22 were resistant and 6 were sensitive to rifampicin. Whereas rifampicin sensitivity profiles of 28 Mycobacterium tuberulosis isolates with probe method showed that 21 were resistant to rifampicin while 7 were sensitive to rifampicin. Taking together overall concordance of probe method with conventional method on LJ media at 64mcg/ml is 96.3% and a discordance of 3.7% Conclusion With the concordance of 96.3% our study showed that indigenously developed molecular probes are rapid and accurate methods for detection of rifampicin resistance in Mycobacterium tuberulosis directly in sputum.

Key words: Molecular probe, Rifampicin resistance, Mycobacterium tuberculosis,Sputum,L J media.



Share this Article


Advertisement
Journal of Contemporary Medical Education

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Suggest a Journal
Publisher Login
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons