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Detection of Caprine-specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction

Asim A. Osman, Imadeldin E. Aradaib, Omer A. Musa.

Abstract
Introduction: This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. Methods: For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 428 base pair (bp) PCR product from milk samples as well as from peripheral blood. Amplification products were visualized on ethidium bromide-stained agarose gels. Results and Discussion: Amplification products were not detected when the PCR was applied to DNA from animal species including cattle, sheep, swine, camel, deer, horse, donkey, and human, which indicates that the 2 pairs of primers are specific for goat. Conclusion: DNA can be extracted from goat milk and would be advantageous in the variety of application such as species identification in milk and milk.

Key words: goat milk; DNA, Rapid detection, PCR assay



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