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Cukurova Med J. 2014; 39(1): 39-47

Revealing the Genetic Variation and Allele Heterozygote Javanese and Arab Families in Malang East Java Indonesia

Nila Kartika Sari, Eriko Prawestiningtyas, Fatchiyah Fatchiyah.

Purpose: The purpose of this study is to identify genetic variability the family of Javanese married with Arab and their allele patterns for paternity testing.
Material and Methods: We used human white blood cell from three generations of three families, consists of:1 grandmother-mother, father-daughter2, grandfather-mother, father-daughter3, grandfather, grandmother mother, father-son. DNA blood samples were isolated by salting out standard procedure, and amplified by PCR with applying 13 CODIS which consists of TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11 and amelogenin primer. The Fingerprint profile was visualized by 8% polyacrylamide gel and took the picture by ChemiDoc gel Imaging and measure the intensity band pattern by Quantity One software
Results: Our result showed that the genetic variability and heterozygote allele increasing by using the 13 CODIS markers from the first generation to the next generation with paternity testing from each family were matched.
Conclusion: We can conclude that in a Javanese-Arab family ethnic seems stimulate the increasing genetic variation and allele heterozygote.

Key words: Javanese Arab Ethnics, DNA fingerprint, 13 CODIS

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