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IJAVMS. 2010; 4(4): 105-113


Optimization of Media and Environmental Conditions for Alkaline Protease Production Using Bacillus Subtilis in Submerged Fermentation Process

Ishtiaq Ahmed, Muhammad Irfan, Muhammad Nadeem, Muhammad Anjum Zia, Bilal Munir Ahmad, Hafiz Muhammad Nasir Iqbal.

Abstract
In the present study Bacillus subtilis was used with rice husk as by-product of rice industry and incorporated with organic salts was employed for the production of protease enzyme using submerged fermentation technique. The main purpose of the project was to study kinetic parameters of protease productivity, its yields and to optimize basal fermentation conditions. After optimization maximum enzyme activity, total protein and cell biomass (216.233.65U/mL), (4.321.11mg/100mL) and (3.950.71g/L) respectively were found on rice husk as a substrate when inoculated with 4% (4 mL/100mL) inoculum size, 7% substrate concentration at pH 11 for 48 hrs fermentation time period and 2% molasses was also used as additional supplement for substrate to get better production of alkaline protease from Bacillus subtilis within shortest and feasible time period. At the end of work it was concluded that due to the maximum production of protease in the presence of cheaper substrate at low concentration, stability at alkaline pH and salt tolerance, these characteristics make the strain and its enzymes useful in different industries.

Key words: Alkaline protease, Bacillus subtilis, Media optimization, Process optimization, Submerged Fermentation Technique



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