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Effect of copper sulfate on the cardiac glycosides accumulation in shoot cultures of Cryptostegia grandiflora

Ahmed Mohamed Ahmed Abd El-Mawla.

Abstract
SUMMARY
AIM: As a part of our continuing interest concerning the production of cardiac glycosides by tissue culture techniques, this study aims to describe for the first time the influence of copper sulfate on the accumulation of cardiac glycosides in shoot cultures of Cryptostegia grandiflora.
METHODS: Shoot cultures of C. grandiflora, inoculated into MS-based culture media supplemented with 0.1 mg L-1 BA, 30 g L-1 sucrose, 0.1 g L-1 myo-inositol and 0.1 g L-1 ascorbic acid were used to study the influence of copper sulfate on the cardiac glycosides content. Cardiac glycosides were quantitatively determined by HPLC analysis. The quantities of cardiac glycosides compounds were estimated on the bases of their area with respective to the area of cryptostigmin II (0.3μg) as external standard.
RESULT: The shoot cultures of C. grandiflora (4 days old) treated with 40 μM copper sulfate for 48 hours showed about 8 and 6 fold increases in the cryptostigmin I and II content respectively in corresponding to untreated shoot cultures. In addition, about 3 fold increases in both cryptostigmin III and IV have been recorded.
CONCLUSION: This experiment indicated the positive significant influence of copper sulfate on the accumulation of cardiac glycosides in shoot cultures of C. grandiflora. This study provided an alternative source for large scale production of cardiac glycosides.

Key words: Keywords: Cryptostegia grandiflora, shoot cultures, copper sulphate, cardiac glycosides, HPLC.



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