Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Anti-diabetic activity of the chloroform extract of Senecio mikanioides Otto in streptozotocin induced diabetic rats

Abeer E. Mahmoud, Mamdouh M. Ali, Hanan S. M. Marzouk.

Cited by (1)

Abstract
Objective: In this study, we examined the effect of oral administration of the chloroform extract of Senecio mikanioides Otto for a period of 30 days at doses of 100, 300 or 500 mg/kg body weight/day on streptozotocin induced diabetic rats.
Methods: Male Sprague-Dawley rats were divided into five groups and classified into; extract treatment group received 100, 300 or 500 mg/kg body weight per day by oral gavage for 30 days; diabetic group administered intraperitoneally a single dose (75 mg/kg body weight) of streptozotocin (STZ); diabetic rats plus tolbutamide group where diabetic rats received the standard hypoglycemic drug tolbutamide (100 mg/kg body weight per day) by oral gavage for 30 days; diabetic rats plus extract group where diabetic rats received the extract (same doses) by gavage for 30 days; as well as a normal group for comparison. In all of these groups, the levels of glucose and insulin were checked in blood, while the levels of reduced glutathione (GSH), nitric oxide (NO), lipid peroxidation (TBARS) and thioredoxin reductase (TrxR) activity were measured in pancreatic tissues.
Results: The results revealed that STZ administration resulted in significant elevation in the level of both TBARS and NO with depletion in the level of GSH as compared with control accompanied with hyperglycemia, hypo-insulinemia and low insulin sensitivity. Moreover, the activity of pancreatic TrxR was lower than the control group. Feeding the diabetic rats for 30 days with the extract normalizes the previous biochemical parameters in dose dependent manner reaching near the tolbutamide treated group at the highest dose.
Conclusion: The chloroform extract of Senecio mikanioides Otto exhibited antidiabetic activity and it corrected the insulin level and its sensitivity in experimentally induced diabetic rats in dose dependent manner. The current results clearly indicated the beneficial effects of the chloroform extract of Senecio mikanioides Otto in both controlling hyperglycemia and the protection of the pancreatic islet cells against oxidative stress in the diabetic animals.

Key words: Blood glucose; Insulin; Lipid peroxidation; Nitric oxide; Senecio mikanioides Otto



Share this Article


Advertisement
Journal of Behavioral Health

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
ScopeMed Home
Follow ScopeMed on Twitter
BiblioCAM
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons