Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access


Vet World. 2009; 2(5): 177-178

Evaluation of a rapid molecular method for detection of Listeria monocytogenes directly from broth culture

R. A. Mathakiya, A. Roy, K. N. Nandasana, P. G. Koringa and C.G. Joshi.

The present study was carried out to know the lowest detection limit of Listeria monocytogenes by PCR. The quantification of organisms was done by CFU counting from ten fold serial dilution and PCR amplification of inlA gene fragment was performed from each dilution. The PCR could detect as low as 20 organisms indicating the lowest PCR detection limit. Thus, lowest number of L. monocytogenes detectable by PCR is a low-cost and rapid procedure that can be appropriated for the detection in real time of low L. monocytogenes levels in naturally contaminated food and is suitable to implement in the food industry.

Key words: Quantification, CFU counting, Serial dilution, PCR, Low-cost, Food industry.

Share this Article

American Journal of Diagnostic Imaging


ScopeMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About ScopeMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Scopemed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.
Scopemed Buttons