Aqueous humour samples of 12 dogs which had undergone surgery for co-existent cataract and ocular hypertension, were collected by anterior chamber centesis, immediately before the corneal incision. The antioxidant enzyme - catalase, was estimated as an indirect measure of free-radical status of the aqueous humour samples of the dogs under study. Since normal aqueous humour value for catalase was lacking, it was compared to the erythrocyte values earlier reported in cataractous dogs, and was found very low. Findings suggest that oxidative changes were happening in the eyes of the present study, resulting in generation of more free-radicals, which would have resulted in cataract.
free-radical, aqueous humour, cataract, dog